Diff Stain Quick
Diff Stain Quick, a rapid method for differentiating (20-30 sec) of corpuscular elements of blood and of Cito-histologic specimens to be examined in optical microscopy.
- Kit consists of 2 fixative solutions 250 ml, 2 solutions red 250 ml and 2 solutions blue from 250 ml.
Procedure of Use:
Methodical staining of cellular types in blood smears and bone marrow. The method foresees the rapid immersion of the slide in order to guarantee an optimum colouring.
1. Preparation of smear on slide.
2. Immerse the slide 5 times for 1-2 seconds in the fixative solution. After each dive wait a moment to eliminate the excess.
3. Immerse the slide 5 times for 1-2 seconds in the red solution. After each dive wait a moment to eliminate the excess.
4. Immerse the slide 5 times for 1-2 seconds in the blue solution. After each dive wait a moment to eliminate the excess.
5. Wash with distilled water or buffered water at PH 7.
6. Let the slide dry in the air.
The final chromatic evaluation of the Diff Stain Quick is comparable to May Grünwald staining: red blood cells will present a violet (clear)/greyish coloration. Neutrophils that are part of Granulocytes will have the blue/violet nucleus, basophils have the nucleus hidden by granules that have been stained with blue, eosinophils such as basophils, have the nucleus hidden by red granules. The lymphocytes that are part of the Agranulociti, have a very large nucleus of blue color, the monocytes are the largest of all and have a blue nucleus.
In the final evaluation it must be considered that the colourings are strongly influenced by the pH of the washing solutions and the dilution buffer, moreover the intensity of the staining can vary according to the time of differentiation.