Occult Blood in the stool Fob Test
The device for the occult blood test in single-phase stool (stool) is a rapid immunocromatographic test for the qualitative detection of human occult blood in the stool.
Many diseases can cause blood hidden in the stool. This is also known as fecal occult blood (FOB), occult blood in human stool or human hemoglobin. In the early stages, gastrointestinal problems such as colon cancer, ulcers, polyps, colitis, diverticulitis and fisses may not show any visible symptoms, only occult blood in the stool. Traditional Guaiac-based methods lack sensitivity and specificity and also have dietary restrictions prior to testing.
The device for the occult blood test in single phase stool (FOB) is a rapid test to detect qualitatively low levels of fecal occult blood. The test uses a double sandwich test of antibodies to selectively detect fecal occult blood at 50 ng/ML. Moreover, unlike the Guaiac test, the precision of the test is not affected by the diet of the patients.
PRINCIPLE OF the METHOD-occult Blood in the stool
The fecal FOB single-phase (stool) occult blood test device is a lateral flow qualitative immunological test for the detection of human occult blood in the stool. The membrane is pre-coated with anti-hemoglobin antibody on the test line area. During the test, the sample reacts with the particle coated with the anti-hemoglobin antibody. Mixture migrate upward on limbs
Na chromatographically for capillary action to react with the anti-hemoglobin antibody on the membrane and generate a coloured line. The presence of this colored line in the Test line area indicates a positive result, while its absence indicates a negative result. To act as a procedural control, a coloured line will always appear in the control line area, indicating that the correct sample volume has been added and that the membrane has been eliminated.
INSTRUCTIONS FOR USE
Carry the test, sample, buffer and/or controls at room temperature (15-30 °c) before testing.
1. For the collection of fecal specimens: Collect the stool in a clean, dry container. You will Get better results if the test is done within 6 hours of the collection. The sample collected can be stored for 3 days at 2-8°c if not tested within 6 hours.
2. To examine fecal specimens: Open the cap of the specimen collection tube, then accidentally poke the specimen collection applicator into the fecal specimen in at least 3 points. Do not lift the fecal specimen. Secure the cap on the specimen collection tube, then shake the tube vigorously to mix the specimen and the extraction buffer. Specimens prepared in the collection tube may be stored up to 6 months at-20 °c if they are not tested within 1 hour of preparation.
3. Bring the packaging to room temperature before opening it. Remove the test from the package and use it within an hour. You will get better results if the test is run immediately after opening the package.
4. Hold the specimen collection tube straight and detach the tip. Reverse The tube and transfer 2 full drops of the extracted sample (approx. 80 μL) into the sample well (S) on the test, then start the timer. Avoid trapping air bubbles in the sample well (S). See illustration below.
5. Read the results at 5 minutes after pouring the sample. Do not read the results after 10 minutes.